Лабораторія біологічних полімерних сполук - ІМВ НАН України 🇺🇦

Head of Department: Maksym Kharhota

E-mail: m.kharkhota@imv.org.ua

Position: Head of the laboratory

Title, degree: PhD in biological sciences

Areas of scientific work of the department

  • Development of methods for isolation, purification and chromatographic separation of compounds using liquid and gas chromatography methods.
  • Development/adaptation of methods of transmission electron microscopy, electron diffraction of crystalline substances or particles.

Keywords: physicochemical methods, gas and liquid chromatography with mass detection, transmission electron microscopy, ultracentrifugation, separation, purification and identification of substances, metabolomics, cell ultrastructure, morphology, electron diffraction

History

The Laboratory of Biological Polymer Compounds was created in 2005 by the merger of the Chemical Analytical Laboratory and the Laboratory of Biophysical Research Methods and is one of the youngest structural divisions of the Institute. Since 1976, the laboratory of biophysical research methods, which has always been managed by K.P. Gushcha, and the chemical analytical laboratory under the leadership of S.M. Veremeychenko, became the support of the Institute's departments and laboratories in the technical implementation of their scientific ideas. The main task of the laboratories was uninterrupted and high-quality performance of work aimed at studying various biological objects and their properties using biophysical and analytical research methods.

Today, the laboratory of biological polymer compounds has combined the equipment park and capabilities of its two predecessors. Available equipment (gas and liquid chromatographs, ultracentrifuges, spectrophotometers, electron microscope, lyophilic drying) and the experience of the laboratory staff allow various analyzes and work with its use.

Scientific work

Thus, laboratory employees perform analyzes of the qualitative and quantitative composition of biologically active compounds, in particular, determination of the amino acid, monosaccharide, fatty acid composition of compounds, determination of mono- and polyhydric alcohols, hydrocarbons, ethylene and acetylene, qualitative and quantitative analysis of phytohormones of various classes, phenolic compounds, flavonoids , water-soluble vitamins, purines and pyrimidines, cyclic dipeptides. Methods of metabolic profiling of microorganisms and plants have been developed. Spectroscopic research methods, lyophilization of microbial material, hydrodynamic methods (analytical and preparative ultracentrifugation) are widely used for sample preparation. The current use of standard methods of transmission electron microscopy with and without casting in epoxy resin, contrast of samples, and electron diffraction of crystalline substances or particles has been established.

Publications

International:

  1. Drobot, K. O., Matvieieva, N. A., Ostapchuk, A. M., Kharkhota, M. A., & Duplij, V. P. (2017). Study of artemisinin and sugar accumulation in Artemisia vulgaris and Artemisia dracunculus “hairy” root cultures. Preparative Biochemistry and Biotechnology, 47(8), 776-781.
  2. Pushkarova, N. O., Kalista, M. S., Kharkhota, M. A., Rakhmetov, D., & Kuchuk, M. V. (2016). Crambe tataria sebeók seeds and plants grown in vitro and in vivo fatty acid composition comparison. Potravinarstvo Slovak Journal of Food Sciences, 10(1), 494-498.
  3. Reva, O. N., Swanevelder, D. Z., Mwita, L. A., Mwakilili, A. D., Muzondiwa, D., Joubert, M., … & Kharkhota, M. A. (2019). Genetic, epigenetic and phenotypic diversity of four Bacillus velezensis strains used for plant protection or as probiotics. Frontiers in Microbiology, 10, 2610.
  4. Rodriguez, R., Konovets, I., Ralchenko, S., Kharkhota, M., Kostyuk, A., Kosach, V., … & Obolenskaya, M. (2021). A low-cost mass spectrometry-based approach for quantifying purines in placental explants. International Journal of Mass Spectrometry, 460, 116490.
  5. Leonova, N., Pirog, T., Piatetska, D., Shevchuk, T., Kharkhota, M., & Iutynska, G. (2020). Synthesisi of gibberellins by surfactant producers Nocardia vaccinii В-7405, Acinetobacter calcoaceticus IMV В-7241 and Phodococcus erytropolis  IMV АС-5017. Scientific Study & Research. Chemistry & Chemical Engineering, Biotechnology, Food Industry, 21(4), 497-509.
  6. Moshynets, O., Babenko, L., Karakhim, S., Kharkhota, M., Kosakivska, I., Rogalsky, S., … & Spiers, A. (2017). N-acyl-L-homoserine lactones are inter-kingdom signalling molecules controlling quorum sensing in the Gram-negative bacteria and priming plants increasing stress adaptation. FEBS Journal, 284(S1), 358-358.
  7. Huliaieva H, Pasichnyk L, Kharchuk M, Kalinichenko A, Patyka V, Bohdan M, Maksin V (2020) Influence of citrates nanoparticles on morphological traits of bacterial cells Pseudomonas syringae pv. atrofaciens. Agriculture & Forestry 66(1): 23-31. 
  8. Vretik LO, Noskov YV, Ogurtsov NA, Nikolaeva OA, Shevchenko AV, Marynin AI, Kharchuk MS, OM Chepurna, Ohulchanskyy TY, Pud AA (2020) Thermosensitive ternary core–shell nanocomposites of polystyrene, poly(N-isopropylacrylamide) and polyaniline. Appl Nanosci 10(12): 4951–4964. 

Ukrainian:

  1.  Huliaieva HB, Tokovenko IP, Pasichnyk LA, Patyka VP, Bohdan MM, Kharchuk MS, Maksin VI, Patyka MV, Kaplunenko VG (2020) Antimicrobial action of vanadium and germanium nanoaquacitrates in vivo and its physiological effect on wheat plants in vitro. Mikrobiol Z 82(6): 43-53.  
  2.  Bulyhina TV, Kyrychenko AM, Kharchuk MS, Varbanets LD (2021) Anti-TMV activities of Pantoea agglomerans lipopolysaccharides in vitro. Mikrobiol Z 83(2):64-72.
  3. Pushkarova, N. O., Kalista, M. S., Kharkhota, M. A., Rakhmetov, D. B., & Kuchuk, M. V. (2016). Biotechnological approaches for conservation of the endangered species Crambe koktebelica (Junge) N. Busch and effect of aseptic in vitro cultivation on its biochemical properties. Biotechnologia Acta, 9(4).
  4. Drobot, K., Matvieieva, N., Kharkhota, М., Shakhovsky, A., & Ratushnyak, Y. (2017). Artemisinin and Total Flavonoid Content in In Vitro Cultivted Artemisia annua L.” Hairy” Root Culture. Agrobiodiversity for Improving Nutrition, Health and Life Quality, (1).
  5.  Паньківська ЮБ, Білявська ЛО, Повниця ОЮ, Загорний ММ, Рагуля АВ, Харчук МС, Загородня СД (2019) Антиаденовірусна активність наночастинок діоксиду титану. Мікробіол Журнал 81(5): 73-84.
  6.  Дерев’янко СВ, Васильченко АВ, Каплуненко ВГ, Головко АМ, Співак МЯ, Харчук МС (2019) Перспективи розробки препаратів для сільського господарства на основі наночастинок. Вісник аграрної науки 10: 44-54.
  7.  Дерев’янко СВ, Решотько ЛМ, Васильченко АВ, Дмитрук ОО, Харчук МС (2019) Токсичність композиції наночастинок неметалів у культурі клітин нирки ембріона свині. Агроекологічний журнал 4: 107-111.
  8.  Tymoshok NO, Kharchuk MS, Kaplunenko VG, Bityutskyy VS, Tsekhmistrenko SI, Tsekhmistrenko OS, Spivak MY, Melnichenko ОМ (2019) Evaluation of effects of selenium nanoparticles on Bacillus subtilis. Regulatory Mechanisms in Biosystems 10(4): 544–552. 
Cooperation

The Center cooperates with various institutes of the National Academy of Sciences of Ukraine, in particular:

 Institute of Bioorganic Chemistry and Petrochemistry named after V.P. Kuhar

Institute of Experimental Pathology, Oncology and Radiobiology named after RE. Kavetsky

Institute of General and Inorganic Chemistry named after V. I. Vernadskyi

Institute of Molecular Biology and Genetics

Institute of Semiconductor Physics named after V.E. Lashkarev

Institute of Cell Biology and Genetic Engineering

Institute of Plant Physiology and Genetics

Institute of Biology and Medicine of Taras Shevchenko National University of Kyiv

Employees of the department

Maksym Kharhota

head of the laboratory, microbiologist, gas and liquid chromatograph operator

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Maksym Serhiyovych Kharchuk

researcher, microbiologist, transmission electron microscope operator

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WoS

Gusha Konstantin Pavlovich

leading engineer, freeze dryer operator

Lyubov Borysivna Gushcha

senior laboratory technician, operator of ultracentrifuges and spectrophotometers

Anna Petrivna Smolenska

laboratory assistant

Polyakova Alina Volodymyrivna

engineer 1 cat.

Awards

1. Kharkhota M.A., Kharchuk M.S. – Award of the President of Ukraine for young scientists (2020)

2. Kharkhota M.A. - Award of the Verkhovna Rada of Ukraine to the most talented young scientists in the field of fundamental and applied research and scientific and technical developments (2013).

3. Kharkhota M.A. – Award of the National Academy of Sciences of Ukraine "Talent, inspiration, work" (2018)