Відділ біології екстремофільних мікроорганізмів - ІМВ НАН України 🇺🇦

Head of department: Oleksandr Borisovych Tashirev

Ph.: +38 (044) 294 69 66

E-mail: tach2007@ukr.net

Position: Head of Department

Title, degree: Prof., Doctor of Technical Sciences

Under supervision of Professor Tashirev O.B. 5 PhD theses were defended.

One doctoral dissertation was prepared for the defense as a result of consulting.

In the department, students of higher educational institutions of Kyiv annually undergo internships and complete course and diploma theses.

Areas of scientific work of the department

  • thermodynamic prediction of the interaction of microorganisms with the main classes of extreme factors (metals, radionuclides, low temperature, synthetic organic substances, etc.);
  • thermodynamic substantiation of the discovery of new extreme types of microorganisms;
  • research of extremophilic microorganisms of natural and man-made ecosystems;
  • development of microbial methods of biofuel production (hydrogen, methane, low molecular weight alcohols) from multicomponent organic waste;
  • modeling of microbial destruction of synthetic organic substances and multicomponent organic waste;
  • detoxification of toxic metal ions and radionuclides;
  • biotechnologies of converting toxic waste into metal concentrate, biofuel and clean water.

You can also learn about the achievements of the Department of Biology of Extremophilic Microorganisms on Youtube-channel.


Keywords: extremophile microorganisms, extreme factors, UV radiation, dehydration, metals, xenobiotics, radionuclides, ecophysiological properties, taxonomy, thermodynamic forecasting, multicomponent organic waste, hydrogen, methane.

History

The department was founded in 1965. For more than 35 years, it was managed by Y.R. Malashenko, corresponding member of the National Academy of Sciences of Ukraine. The main scientific directions of the department were fundamental and applied studies of methane-oxidizing bacteria (methanotrophs). During this period, the ecology of methanotrophs, their taxonomy, physiology and biochemistry was studied. Since 2005, the department has been headed by Doctor of Technical Sciences, Prof. O.B. Tashyrev, the priority direction of the department is the study of extremophilic microorganisms and the development of environmental protection and bioenergy biotechnologies for the purification of ecosystems from concentrated organic waste, toxic metals and radionuclides with the production of valuable products (hydrogen, methane, metal concentrate, biofertilizer, purified water).

Scientific work

The department studies extremophilic microorganisms of natural and man-made ecosystems. These include western Antarctica (phytocenoses, ornithogenic soil, freshwater lakes, rocks, etc.), soil of the Negev desert (Israel), soil and silt on the coast of the Dead Sea (Israel), karst caves (Ukraine), silt of hypersaline lakes (Crimea and Bulgaria), bottom sediments of the Black Sea, littoral and deep lake samples. Baikal (Russian Federation), phytocenoses and volcanic emissions of a high-altitude region (Ecuador), clays of karst cavities (Kuibyshevska mine, Optimistychna cave, Mlynky, Ozerna, Atlantis, etc.) The study of extremophilic microorganisms concerns their ecology, biodiversity, physiology, systematics, resistance to extreme factors (γ-radiation, UV radiation, toxic metals, low temperatures, etc.).

From 40 to 70% of the studied Antarctic microorganisms, regardless of the temperature regime of their release, are able to grow in a wide temperature range from 1°С to 30°С, that is, they are psychrotolerant. In selective conditions (1°С or 5°С), psychrophilic Antarctic bacteria and yeast were isolated, which grew in the range from 1°С to 18°С and did not grow at a temperature of 30°С. It has been shown that Antarctic microorganisms are able to survive in extreme conditions of high UV radiation. For most bacteria, LD99,99 was in the range of ~200–500 J/m2, the exceptions were bacteria of the genera: Pseudomonas, Serratia, Frondihabitans. About 50% of Antarctic bacteria are resistant to dehydration. There is a correlation between the resistance of bacteria to dehydration and UV radiation. Antarctic yeast strains were found to be more resistant to UV than bacteria. Pigmented strains, were the most resistant to UV among yeasts, LD99,99 for them varied between 600-1600 J/m2. Resistance to UV radiation was found in all studied bacteria from the hypersaline ecosystems of the Dead Sea, Crimea, and high-altitude ecosystems of Ecuador. Lethal doses of UV (LD90 and LD99,99) for spore-forming strains of the genus Bacillus were 100-170 and 1100-1500 J/m, respectively2.

It is theoretically justified and experimentally confirmed that the stability of microorganisms of natural ecosystems is a general biological phenomenon. The presence of microorganisms resistant to extremely high concentrations of toxic metals (500-1000 mg/l Cu2+, Co2+, Ni2+, CrO42-).

Studying the metal resistance of microorganisms, 17 bacterial cultures of the rhizosphere of wheat were isolated Triticum durum (variety Benefit), which were resistant to cadmium concentrations in the range of 11.2-100 mg/l Cd2+.  All studied strains were highly resistant to Cu2+, Co2+, Ni2+, CrO42- in liquid and agar media. The maximum allowable concentrations of metal ions were hundreds of mg/l, which is one or two orders of magnitude higher than generally recognized bactericidal concentrations. The genomes of 6 strains were annotated: Brevundimonas vesicularis USM1, Pseudoarthrobacter oxydans USM2, Pseudomonas lini USM3, Pseudomonas putida USM4, Cupriavidus gilardii USM5, Cupriavidus taiwanensis USM6. According to the genome analysis, it was established that all the studied strains have common pathways and enzymes for the degradation of cyclic compounds. The highest number of genes was represented for benzoate degradation by hydroxylation, 1,4-dichlorobenzene degradation, and the geraniol degradation pathway. Strain Cupriavidus taiwanensis USM6 showed the greatest resistance to toxic metals. The presence of the heavy metal efflux mechanism of the CzcA family, cationic ATPase CzcP, and other proteins determined resistance to Cd2+, Cu2+, Co2+ and Ni2+ in high concentrations (100-500 mg/l).

A separate direction of the department is bioprospecting - a purposeful search in extreme ecosystems for microorganisms with promising biotechnological properties. A collection of extremophilic microorganisms (bacteria and yeast) with unique properties was created using the bioprospecting approach over 10 years. They are resistant to 100-50,000 mg/l of copper, chromium, cadmium, nickel and mercury ions, and are capable of removing them from aqueous solutions. UV-resistant yeast are hyperproducers of melanin and carotenes; the mass of pigments is 10-12% of the total mass of the cell.

Melanin and carotene producing strains

As a result of the phylogenetic and phenotypic analysis, the taxonomic position of microorganisms was determined. Representatives were found among Antarctic bacteria Proteobacteria (genus: Pseudomonas, Stenotrophomonas,Brevundimonas,Serratia), Actinobacteria (genus: Frondihabitans,Microbacterium,Rhodococcus, Arthrobacter,Micrococcus,Rothia), Bacteroidetes (genus Sphingobacterium), Firmicutes (genus:Sporosarcina, Staphylococcus,Bacillus). Antarctic yeast is represented Basidiomycota (genus: Tremella,Rhodosporidium,Cryptococcus,Leucosporidiella, Leucosporidium,Rhodotorula) and Ascomycota (genus: Candida, Exophiala, Debaryomyces). Microorganisms isolated from the hypersaline ecosystems of the coast of the Dead Sea and Crimean lakes are presented Firmicutes (genus: Bacillus, Staphylococcus), from the soil of the Negev Desert - Actinobacteria (genus: Kocuria, Micrococcus), from clays of karst cavities - Actinobacteria (genus: Microbacterium, Rhodococcus, Arthrobacter) and Proteobacteria (genus: Pseudomonas). Nucleotide sequences of 16S rRNA, 18S rRNA and 26S rRNA genes have been deposited in the International GenBank database under the numbers HG421018, HG796183–HG796195, HG315621–HG315625, HG518622–HG518626, KF181970–KF181975, LT220850–LT22080.

A line for the production of dry granular microbial preparation (DMP) was established, the use of which ensures the effective destruction of multi-component solid and liquid food waste with the production of an environmentally clean energy carrier - molecular hydrogen.

General view of the granular microbial preparation

The resulting granular microbial preparation has the following characteristics:

  • the preparation contains groups of highly active hydrogen-synthesizing microorganisms;
  • speed and ease of manufacture;
  • high rate of activation of microorganisms of the preparation;
  • versatility and a wide range of substrates that can be used.

Scaling and optimization of the food waste fermentation process was carried out in laboratory cultivators with a volume of 0.5 l, later - in a laboratory fermenter with a volume of 20 l, and further - in a research and industrial installation with a volume of 240 l. As a result, the optimal values of pH (6.0...7.0) and Eh (-250...-350 mV) were determined of the media for synthesis of H2. The engineering and technological parameters of the functioning of the laboratory fermenter with a volume of 20 liters have been optimized: the speed of mixing the fermentation mixture is 24 revolutions/min.; mixing mode – 10 minutes of mixing / 20 minutes of pause; the weight ratio of the solid phase (waste) to the liquid (water) is 1:3. As a result, high efficiency of molecular hydrogen synthesis and waste destruction was achieved. Yield of H2 (VH2) was 100-123 l/kg of waste, the destruction time (T) was only 3-4 days, and the coefficient of waste destruction Kd = 90-94. ETP operation of the 240 L installation was obtained, such as the ratio of solid and liquid phases - 1:4 and the mass transfer mode - 0.5 min of mixing / 60 min of pause. Under such conditions, the duration of the technological fermentation cycle (T) was 1.5 days. Waste destruction coefficient (Kd) reached 86. The yield of hydrogen was 45 l from 1 kg of waste in terms of absolutely dry mass. The maximum concentration of hydrogen (Н2max) was 50%. The possibility of using unfermented residues of multicomponent food waste as a highly effective fertilizer is shown. The obtained modified lignin cellulose (MLC) can be prospectively used as a preparation that increases the fertility of depleted soils.


Research and industrial plant for fermentation of waste (1 – working chamber of the fermenter; 2 – motor for mass exchange (mixing) of waste; 3 – hose for closed mass exchange (reciprocating pumping) of the fermentation mixture; 4 – fittings for introduction of regulators, selection of culture liquid and removal of synthesized gas)

The study of microorganisms during the hydrogen fermentation of multicomponent food waste using HMP showed the presence of three physiological groups in the composition of the drug (CFU/g): aerobic - n×102 – n×104, facultatively anaerobic - n×102 – n×103 and obligately anaerobic - n×101 – n×102. During fermentation, the number of microorganisms increased within n×105 – n×107 CFU/ml in the final phase of fermentation. 27 strains involved in fermentation were isolated and characterized. Among them, based on the results of 16S rRNA gene sequencing and phylogenetic analysis, 19 strains were classified as species of the genus Bacillus, and 8 strains - to the genus Clostridium.

Conferences
  1. International scientific conference "Microbiology and immunology - development prospects in the 21st century" (April 10-11, 2014, Kyiv, Ukraine).
  2. XXI International Conference of Students, Graduate Students and Young Scientists "Lomonosov-2014" (April 7-11, 2014, Moscow, Russia).
  3. IX International Conference of Young Scientists "Biology: From the Molecule to the Biosphere" (November 18-21, 2014, Kharkiv, Ukraine).
  4. Scientific and theoretical conference of Irkutsk State University (April 2, 2014, Irkutsk, Russia).
  5. 10th International Congress on Extremophiles (September 7-11, 2014, Saint Petersburg, Russia).
  6. Conference “Jacques Benveniste: Ten Years After. A Glimpse at the Future of Biology and Medicine” (October 3, 2014, Roma, Italy).
  7. International Scientific and Practical Conference "Biological Nitrogen Fixation" (October 7-11, 2014, Ternopil, Ukraine).
  8. XVIII International scientific and practical conference of students, postgraduates and young scientists "Ecology. Man. Society" (2015, Kyiv, Ukraine).
  9. International conference for young scientists “Actual problems of microbiology and biotechnology” (June 1-4, 2015, Odessa, Ukraine).
  10.  The III International Scientific and Practical Conference is dedicated to the 10th anniversary of the Department of Biotechnology of the National Aviation University and the 175th anniversary of the Department of Pharmacology of the National Medical University named after O.O. Bogomolets (October 22-23, 2015, Kyiv, Ukraine).
  11.  X International Conference of Young Scientists "Biology: From the Molecule to the Biosphere" (December 2-4, 2015, Kharkiv, Ukraine).
  12.  1st International Conference on Recent Advances in Bioenergy Research, Sardar Swaran Singh National Institute of Renewable Energy, (Mar 14-17, 2015, Kapurthala, India).
  13.  II International scientific conference “Microbiology and immunology – the development outlook in the 21st century” (April 14-15, 2016, Kyiv, Ukraine).
  14.  International Scientific Conference "Achievements and Development Prospects of Microbiology" (October 12-14, 2016, Lviv, Ukraine).
  15.  34th SCAR Open Science Conference (August 20-30, 2016, Kuala Lumpur, Malaysia).
  16.  Scientific reporting session. Targeted comprehensive program (TCP) of scientific research of the National Academy of Sciences of Ukraine "Fundamental aspects of renewable hydrogen energy and fuel cell technologies" (2016, Kyiv, Ukraine).
  17.  Fifth International Conference "Ecological Engineering and Environmental Protection" (June 5-7, 2017, Plovdiv, Bulgaria).
  18.  7th International Weigl conference (September 26-29, 2017 Lviv, Ukraine).
  19.  International Antarctic conference (September 21, 2017, Quito, Ecuador).
  20.  7th Congress of European Microbiologists (July 9-13, 2017, Valencia, Spain).
  21.  XV Congress of the Society of Microbiologists of Ukraine named after S.M. Vinogradsky (September 11-15, 2017, Odesa, Ukraine).
  22.  2nd International Conference „Smart Bio“ (May 03-05, 2018, Kaunas, Lithuania).
  23.  International Conference on the Application of Microorganisms for the Radioactive Waste Treatment (May 18, 2018, Busan, Korea).
  24.  The fourth China-Ukraine forum on science and technology (September 14-20, 2018, Harbin, China).
  25.  XII Scientific Conference with International Participation „Ecology and Health” (June 07-09, 2018, Plovdiv, Bulgaria).
  26.  Seminar in Laboratori Nazionali di Frascati of the Istituto Nazionale di Fisica Nucleare (July 16-20, 2018, Frascati, Italy).
  27.  International Conference Advances in Microbiology and Biotechnology (October 29-31, 2018, Lviv, Ukraine).
  28.  Annual Conference 2018 of the Association for General and Applied Microbiology (April 15-18, 2018, Wolfsburg, Germany).
  29.  17th International Symposium on Microbial Ecology (August 12-17, 2018, Leipzig, Germany).
  30.  XIII International Scientific Conference "Factors of Experimental Evolution of Organisms" (September 17-21, 2018, Yaremche, Ukraine).
  31.  3rd International Conference „Smart Bio“ (May 02-04, 2019, Kaunas, Lithuania).
  32.  Sixth International Conference with Youth Scientific Session “Ecological engineering and environment protection” (June 5-7, 2019, Burgas, Bulgaria).
  33.  8th Congress of European Microbiologists (July 7-11, 2019, Glasgow, Scotland).
  34.  V All-Polish scientific and practical conference "Renewable energy sources - theory and practice" (October 9-11, 2019, Opole, Poland).
  35.  Scientific reporting session. Targeted comprehensive program of scientific research of NASU "Development of scientific principles of obtaining, storing and using hydrogen in autonomous energy supply systems" (December 11, 2019, Kyiv, Ukraine).
  36.  XXI International Scientific and Practical Conference. ECOLOGY. MAN. SOCIETY (May 21-22, 2020, Kyiv, Ukraine).
  37.  IV International scientific and practical conference dedicated to the 15th anniversary of the Department of Biotechnology of NAU (September 23, 2020, Kyiv, Ukraine).

Publications
2021

International

  1. Hovorukha Vira, Olesia Havryliuk, Galina Gladka, Oleksandr Tashyrev, Antonina Kalinichenko, Monika Sporek, Agnieszka Dołhańczuk-Śródka Hydrogen dark fermentation for degradation of solid and liquid food waste. Energies. 2021; 14(7):1831. https://doi.org/10.3390/en14071831
  2. Gladka Galina, Vira Hovorukha, Victoria Romanovskaya, Oleksandr Tashyrev. Correlation Between Resistance to UV Irradiation and the Taxonomic Position of Microorganisms.  Environmental Research, Engineering and Management. 2021; 77(1):67-75.  DOI 10.5755/j01.erem.77.1.23832
  3. Vira Hovorukha, Olesia Havryliuk, Galyna Gladka, Bida Iryna, Yanina Danko, Oleksandra Shabliy, Oleksandr Tashyrev. Gaseous fuel obtaining via fermentation of organic landfill waste. Ecological Engineering and Environment Protection. 2020; 1:36-48.

Ukrainian

  1. Havryliuk OA, Hovorukha VM, Sachko AV, Gladka GV, Tashyrev OB. Quantitative indicators of copper-resistant microorganisms distribution in natural ecosystems. Biotechnologia Acta. 2021; 14(1):69-80. https://doi.org/10.15407/biotech14.01.69
  2. Borzova NV, Gladka GV, Gudzenko OV, Hovorukha VM, Tashyrev ОB. Enzymatic activity of psychrotolerant antarctic bacteria. Mikrobiol. Zhurnal. 2021; 83(2):3-11.
2020

International

  1. Tashyrev O, Hovorukha V, Shevel V, Havryliuk O, Sioma I. Development of novel universal biotechnologies for obtaining valuable products from a wide range of wastes. Ecological Engineering and Environment Protection. 2020; 1:5-17. doi.org/10.32006/eeep.2020.1.0517
  2. Havryliuk Olesia, Vira Hovorukha, Galina Gladka, Oleksandr Tashyrev. Bioremoval of copper(ii) via hydrogen fermentation of Ecologically hazardous multicomponent food waste. Ecological Engineering and Environment Protection. 2020; 2:5-14. doi.org/10.32006/eeep.2020.1.0514
  3. Vira Hovorukha, Oleksandr Tashyrev, Olesia Havryliuk, Larysa Iastremska High Efficiency of Food Waste Fermentation and Biohydrogen Production in Experimental-industrial Anaerobic Batch Reactor. Open Agriculture Journal. 2020; 14:174-186. https://benthamopen.com/EPUB/BMS-TOASJ-2019-HT1-1140-2
  4. Olesia Havryliuk, Vira Hovorukha, Marianna Patrauchan, Noha H. Youssef, Oleksandr Tashyrev. Draft whole genome sequence for four highly copper resistant soil isolates Pseudomonas lactis strain UKR1, Pseudomonas panacis strain UKR2, and Pseudomonas veronii strains UKR3 and UKR4. Current Research in Microbial Sciences. 2020; 1:44-52. https://doi.org/10.1016/j.crmicr.2020.06.002
  5. Hovorukha V, Bhattacharyya A, Iungin O, Tashyreva H, Romanovska V, Havryliuk O, etc. Draft genome sequences of six strains isolated from the rhizosphere of wheat grown in cadmium-contaminated soil. Microbiology Resource Announcements. 2020; 9(34):e00676-20. https://doi.org/10.1128/MRA.00676-20

Ukrainian

  1. Hovorukha VM, Havryliuk OA, Gladka GV, Tashyrev OB. The bioremoval of toxic chromium (VI) via dark hydrogen fermentation of multicomponent organic waste. Biotechnologia Acta. 2020; 13(4):49-59. https://doi.org/10.15407/biotech13.04.049
2019

International

  1. Govorukha Vira, Tashyrev Oleksandr, Shevel Valery. Novel biotechnologies for purification of radioactive wastewater. Journal of condensed matter nuclear science. 2019; 28: 53-55. https://www.lenr-canr.org/acrobat/BiberianJPjcondensedza.pdf
  2. Tashyrev Oleksandr, Govorukha Vira, Matvieieva Nadiia, Havryliuk Olesia. Thermodynamic prognosis for novel environmental biotechnologies of radioactive waste water purification. Journal of condensed matter nuclear science. 2019; 28:50-52. https://www.lenr-canr.org/acrobat/BiberianJPjcondensedza.pdf
  3. Hovorukha V, Tashyrev O, Tashyreva H, Havryliuk O, Bielikova O, Iastremska L. Increase in efficiency of hydrogen production by optimization of food waste fermentation parameters. Energetika. 2019; 65(1):85-94.  https://doi.org/10.6001/energetika.v65i1.3977

Ukrainian

  1. Tashyrev OB, Sioma IB, Tashyreva GO, Hovorukha VM. Bromthymol blau as the universal indicator for determining the stereometric allocation of pH and Eh in the medium in heterophase microorganisms cultivation. Mikrobiol. Zhurnal. 2019; 81(2):14-24. doi: https://doi.org/10.15407/microbiolj81.02.014
  2. Матвєєва НА, Гаврилюк ОА, Дуплій ВП. Вплив ванадію (IV) на ріст «бородатих» коренів Artemisia tilesii Ledeb. Фактори експериментальної еволюції організмів. 2019; 25:276-280. https://doi.org/10.7124/FEEO.v25.1177
  3. Борзова НВ, Гудзенко ЕВ, Гладка ГВ, Варбанец ЛД, Таширев АБ. Аутэкология и гидролитическая активность микроорганизмов наземных экосистем Антарктики, Эквадора и Израиля. Мікробіол. журнал. 2019; 81(4):29-41. DOI: 10.15407/microbiolj81.04.029
  4. Борзова НВ, Гудзенко ЕВ, Гладка ГВ, Варбанец ЛД, Таширев АБ. Энзиматическая активность дрожжей Антарктического региона. Мікробіол. журнал. 2019; 81(6): 16-29. DOI: 10.15407/microbiolj81.06
2018

International

  1. Hovorukha VM, Tashyrev OB, Matvieieva NA, Tashyreva HO, Havryliuk OA, Bielikova O. Iu., Sioma IB. Integrated approach for development of environmental biotechnologies for treatment of solid organic waste and obtaining of biohydrogen and lignocellulosic substrate. Environmental Research, Engineering and Management. 2018; 74(4):31-42. http://dx.doi.org/10.5755/j01.erem.74.4.20723
  2. Iungin O, Govorukha V, Tashyrev O. Rhizospheric bacteria for destruction of nitrochloroaromatic compounds. Journal of Environmental Research, Engineering and Management. 2018; 74(3):80-86. https://doi.org/10.5755/j01.erem.74.3.21069
  3. Oleksandr Tashyrev, Vira Hovorukha, Olga Suslova, Hanna Tashyreva Thermodynamic prediction for development of novel environmental biotechnologies and valuable products from waste obtaining. Ecological Engineering and Environment Protection. 2018; 1:24-35. 
  4. Hovorukha Vira, Havryliuk Olesia, Tashyreva Hanna, Tashyrev Oleksandr, Sioma Iryna. Thermodynamic substantiation of integral mechanisms of microbial interaction with metal. Ecological Engineering and Environment Protection. 2018; 2:55-63. 
  5. Oleksandr Tashyrev, Nadiia Matvieieva, Vira Hovorukha, Hanna Tashyreva, Olena Bielikova, Olesia Havryliuk, Volodymyr Duplij. Application of lignocellulosic substrate obtained after hydrogen dark fermentation of food waste as biofertilizer. Industrial Biotechnology. 2018; 14(6):315-322.
  6. Мatvieieva N, Havryliuk O, Duplij V, Drobot K. The Effect of High Temperature on the Flavonoid Accumulation in Artemisia “Hairy” Roots. Agr. bio. div. Impr. Nut., Health Life Qual. 2018; 262-267. https://doi.org/10.15414/agrobiodiversity.2018.2585-8246.262-267

Ukrainian

  1. Юнгін ОС. Антифунгальні агенти у розрізі набутої резистентності у грибів роду Candida. Вісник проблем біології і медицини. 2018; 2(144):83-86. http://nbuv.gov.ua/UJRN/Vpbm_2018_2_20
  2. Борзова НВ, Гладка ГВ, Варбанец ЛД, Таширев АБ. β-маннаназная активность дрожжей, выделенных в Антарктике. Мікробіол. журнал. 2018; 80(2):28-43. https://doi.org/10.15407/microbiolj80.02.028
  3. Юнгин ОС, Беликова ЕЮ, Гладка ГВ, Таширев АБ. Генетический потенциал бактерий, выделенных из загрязненных кадмием почв. В зб.: XIII Міжнародної наукової конференції «Фактори експериментальної еволюції організмів». 2018; 23:352-6. DOI: https://doi.org/10.7124/FEEO.v23.1040
  4. Гладка ГВ, Романовська ВО, Белькова НЛ, Юнгін ОС, Таширев ОБ. Таксономічне положення пігментованих дріжджів, ізольованих з екосистем Антарктики В зб.: XIII Міжнародної наукової конференції «Фактори експериментальної еволюції організмів». 2018; 23:285-290. DOI: https://doi.org/10.7124/FEEO.v23.1029
  5. Бєлікова ОЮ, Матвєєва НА, Ястремська ЛС, Таширев ОБ. Визначення стійкості азотфіксувальних мікроорганізмів ґрунту Еквадору до токсичних металів (Cro42–, Ni2+, Cu2+). В зб.: XIII Міжнародної наукової конференції «Фактори експериментальної еволюції організмів». 2018; 23:267-272. DOI: https://doi.org/10.7124/FEEO.v23.1026
  6. Матвєєва НА, Гаврилюк ОА, Ястремська ЛС. Вплив зниженої температури на синтез флавоноїдів у культурах «бородатих» коренів  Artemisia vulgaris and Artemisi aannua. В зб.: XIII Міжнародної наукової конференції «Фактори експериментальної еволюції організмів». 2018; 23:302-307. DOI: https://doi.org/10.7124/FEEO.v23.1032
  7. HovorukhaVM, TashyrevOB. Thermodynamic prognosis of the efficiency of toxic metals extraction from the solution by microorganisms and their genetic potential. Збірник наукових праць «Фактори експериментальної еволюції організмів». 2018; 23:357-363. DOI: https://doi.org/10.7124/FEEO.v23.1041
  8. Сіома ІБ, Говоруха ВМ, Таширев ОБ. Ртуть-резистентні бактерії у   екосистемах Антарктики. Збірник наукових праць «Фактори експериментальної еволюції організмів». 2018; 23:381-387. DOI: https://doi.org/10.7124/FEEO.v23.1045
  9. Гаврилюк ОА, Говоруха ВМ, Таширев ОБ. Стійкість мікроорганізмів чорноземного грунту до розчинних сполук міді. Збірник наукових праць «Фактори експериментальної еволюції організмів». 2018; 23:273-279. DOI: https://doi.org/10.7124/FEEO.v23.1027
  10. Tashyrev OB, Sioma IB, Tashyreva GO, Hovorukha VM. Natural and synthetic solid carriers in flow module for microbial sewage filtrate purification. Biotechnologia Acta. 2018; 11(6):73-81.  https://doi.org/10.15407/biotech11.06.073
  11. Bilyk T, Lukianenko N, Vityk K, Havryliuk O. Application of plant essential oils for improving air quality in the lecture-halls of universities. Proceedings of the National Aviation University. 2018; 2(75):60-66.  DOI: 10.18372/2306-1472.75.13119
2017

International

  1. Sioma IB, Tashyrev AB, Govorukha VM, Prekrasna YP.Toxic metals extraction during potato fermentation. Ecological Engineering and Environment Protection. 2017; 1:62-67. http://ecoleng.org/archive/2017/1/62-67.pdf
  2. Matvieieva NA, Churkina LN, Shakhovsky AM, Kadurin SV, Tashyrev AB.Study of resistence to antibiotics of microorganisms isolated from Antarctic cliffs and Blask Sea bottom sediments. Ecological Engineering and Environment Protection. 2017; 1:68-72. http://ecoleng.org/archive/eContents8.2017.html#9
  3. Hanna Tashyreva, Oleksandr Tashyrev, Vira Govorukha, Olesya Havryliuk. The effect of mixing modes on biohydrogen yield and spatial pH gradient at dark fermentation of solid food waste. Ecological Engineering and Environment Protection. 2017; 2:53-62. http://ecoleng.org/archive/2017/2/53-62.pdf

Ukrainian

  1. Бабенко ЛМ, Мошинец ЕВ, Рогальский СП, Щербатюк НН, Суслова ОС, Косаковская ИВ. Влияние предпосевного праймирования N-гексаноил-L-гомосеринлактоном на формирование ризосферной микрофлоры и структуру урожайности Triticum aestivum L. Вісник Харківського національного аграрного університету. Серія: Біологія. 2017; 1:106-118. http://nbuv.gov.ua/UJRN/Vkhnau_biol_2017_1_13.
  2. Tashyrev O, Romanovskaya V, Rokitko P, Tashyreva H, Prytula I, Suslova O, Govorukha V, Prekrasna Ie, Gladka G. Аutecology and taxonomy of bacteria of extremal environments. Мікробіол. журнал. 2017; 79(1):100-113. http://nbuv.gov.ua/UJRN/MicroBiol_2017_79_1_11
  3. Зеленая ПП, Гладка ГВ, Шепелевич ВВ, Юмына ЮМ, Сенчило НВ, Скивка ЛМ.Чувствительность к ультрафиолетовому излучению грамотрицательных эпифитных бактерий из 10 км зоны отчуждения ЧАЭС. Мікробіологія і біотехнологія. 2017; 37(1): 94-104. http://nbuv.gov.ua/UJRN/MiB_2017_1_11
  4. Belikova O, Matvieieva N, Tashyrev O. Toxicity of unfermented waste residue obtained in microbial destruction of organic waste. Агроекологічний журнал. 2017; 1:128-130. http://nbuv.gov.ua/UJRN/agrog_2017_1_22
  5. Романовская ВА, Белькова НЛ, Парфенова ВВ, Гладка ГВ, Мучник ФВ, Таширев АБ. Филогенетический анализ коллекционных штаммов метанокисляющих бактерій. Мікробіол. журнал. 2017; 79(2):3-12. http://nbuv.gov.ua/UJRN/MicroBiol_2017_79_2_2
2016

International

  1. Galach’yants AD, Bel’kova NL, Sukhanova EV, Romanovskaya VA, Gladka GV, Bedoshvili ED,  Parfenova VV.  Diversity and Physiological and Biochemical Properties of Heterotrophic Bacteria Isolated from Lake Baikal Neuston. Microbiology. 2016; 85(5):604-613. https://link.springer.com/article/10.1134/S0026261716050064.

Ukrainian

  1. Романовська ВО, Рокитко ПВ, Гладка ГВ, Таширев ОБ. Стійкість до дегідратації екстремофільних бактерій з Антарктики та гіперсолоних водойм. Мікробіол. журнал. 2016; 78(2):74-79.
  2. Govorukha VM, Tashyrev OB. The regularities of iron compounds transformation by Citrobacter freundii Ml-31.1/1. Mikrobiol. Zhurnal. 2016; 78:33-43 doi: https://doi.org/10.15407/microbiolj78.01.033.
2015

International

  1. Govorukha V, Radchenko O, Tashyrev O. Thermodynamic prognosis of microbial interaction with iron compounds. Ecological Engineering and Environment Protection. 2015; 1:12-23. http://ecoleng.org/archive/2015/1/12-23.pdf.
  2.  Suslova OS, Rokitko PV, Bondar KM, Golubenko OO, Tashyrev AB. Biochemical mechanisms of resistance to p-nitrochlorobenzene of karst cave microorganisms. Ukr. Biochem. J. 2015; 4:32-36. https://doi.org/10.15407/ubj87.04.032
  3. Matvieieva N, Gladka G, Govorukha V, Prekrasna Ie, Suslova O, Tashyrev O. Antimicrobial activity of extracts from Ruta graveolens L. “hairy” roots. Agrobiodiversity for improving nutrition, health and life quality. Part ІІ. Publisher Slovak University of Agriculutre. Nitra 2015;468-470.
  4. Hamilton R, Kits KD, Romanovskaya VA, Rozova ON, Yurimoto H, Iguchi H, Khmelenina VN, Sakai Y, Dunfield PF, Klotz MG, Knief C, Op den Camp HJM, Jetten MSM, Bringel F, Vuilleumier S, Svenning MM, Shapiro N, Woyke T, Trotsenko YA, Stein LY, Kalyuzhnaya MG. Draft genomes of gammaproteobacterial methanotrophs isolated from terrestrial ecosystems. Genome Announcements.  2015; 3(3):e00515-15. doi:10.1128/genomeA.00515-15.

Ukrainian

  1.  Таширев АБ, Рокитко ПВ, Суслова ОС. Устойчивость микроорганизмов карстовых полостей Мушкарова Яма и Куйбышевская к соединениям токсичной меди(II). ВОДА: ХИМИЯ и ЭКОЛОГИЯ. 2015; 79(1):64–72. https://elibrary.ru/contents.asp?issueid=1397987.
  2.  Govorukha VM, Havrylyuk OA, Tashyrev OB. Regularities of quantitative distribution for Fe(III)-reducing bacteria in natural ecosystems. Biotechnologia Acta. 2015;8(3):123-128. DOI: 10.15407/biotech8.03.123.
  3. Tashyrev OB, Prekrasna IeP, Tashyreva GO. Resistance of microbial communities of Ecuador ecosystems to representative toxic metals – CrO42-, Co2+, Ni2+, Cu2+, Hg2+. Мікробіол. журнал. 2015; 77(4):46-61.
  4.  Tashyrev OB, Govorukha VM. The widespread of Fe(III)-reducing bacteria in natural ecosystems of Ecuador.  Мікробіол. журнал. 2015; 77(4):62-68. http://nbuv.gov.ua/UJRN/MicroBiol_2015_77_4_9
  5.  Suslova OS, Rokitko PV, Bondar KM, Golubenko OO, Tashyrev AB. Resistance of karst caves microorganisms to p-nitrochlorobenzene. Biotechnologia Acta. 2015; 8(4):135-140. DOI: 10.15407/biotech8.04.135
  6.  Tashyrev OB, Suslova OS, Rokitko PV. The effect of p-nitrochlozobenzene on homeostasis quantitative parameters on karst cave clays and Equador soils microbial communities. Mikrobiol. Zhurnal.  2015; 4:38-44.
  7. Prekrasna IeP, Tashyrev OB. Copper resistant strain Candida tropicalis RomCu5 interaction with soluble and insoluble copper compounds. Biotechnologia Acta. 2015; 8(5):93-102. DOI: 10.15407/biotech8.05.093
  8.  Gladka GV, Romanovskaya VA, Tashyreva HO, Tashyrev OB. Phylogenetic analysis and autecology of spore-forming bacteria from hypersaline environments. Mikrobiol. zhurnal. 2015; 77(6):31-38. doi: https://doi.org/10.15407/microbiolj77.06.031
  9. Сіома ІБ, Таширев ОБ. Знешкодження токсичного хромату при збродженні екологічно небезпечних харчових відходів грунтовими мікроорганізмами. Вісник аграрної науки. 2015. 2015; 7:49-53. http://nbuv.gov.ua/UJRN/vaan_2015_7_12.
  10.  Таширев ОБ, Притула ІР, Таширева ГО. Ідентифікація споротвірних бактерій в асоціаціях воденьутворюючих мікроорганізмів. Вісник аграрної науки. 2015; 15(3):41-46.
  11. Govorukha VM, Tashyrev OB. Regulation of interaction of the strain citrobacter freundii ml-31.1/1 with iron compounds. Biotechnologia acta. 2015; 8(5):103-111.
  12. Говоруха ВМ, Таширев ОБ. Динаміка взаємодії асоціації спороутворюючих ґрунтових мікроорганізмів з Fe(III). Вісник аграрної науки.  2015; 9:62-65.
2014

International

  1. Victoria Romanovskaya, Galina Gladka, Oleksandr Tashyrev. Autecology of microorganisms of coastal ecosystems of the Dead Sea. Ecological Engineering and Environment Protection. 2014; 1:44-49.
  2. Suslova Оlga, Govorukha Vera, Brovarskaya Оksana,  Matveeva Nadezhda, Tashyreva Hanna, Tashyrev Oleksandr. Method for Determining Organic Compound Concentration in Biological Systems by Permanganate Redox Titration. Int. J. Bioautomation. 2014; 18(1):45-52.
  3. Vasileva-Tonkova Evgenia, Romanovskaya Victoria, Gladka Galina,  Gouliamova Dilnora, Tomova Iva, Stoilova-Disheva Margarita, Tashyrev Oleksandr. Ecophysiological properties of cultivable heterotrophic bacteria and yeasts dominating in phytocenoses of Galindez Island, maritime Antarctica. World J. Microbiol. Biotechnol. 2014; 30(4):1387-1398. doi: 10.1007/s11274-013-1555-2.
  4. Tomova I, Gladka G, Tashyrev A, Vasileva-Tonkova E. Isolation, identification and hydrolytic enzymes production of aerobic heterotrophic bacteria from two Antarctic islands. International journal of environmental sciences. 2014; 4(5):614-625.
  5. Tashyreva A, Tashyrev O, Prytula I. The Novel Comprehensive Approach for Agricultural and Landfill Biomass Microbial Fermentation and Biogas Production. Biotechnology and Plant Breeding Perspectives. 2014; 347-356.
  6. Oleksandr Tashyrev, Ievgeniia Prekrasna. Express Method for Redox Potential and Ph Measuring in Microbial Cultures. Int. J. Bіоautomation. 2014; 18(3):217-230.
  7. Романовская ВА, Парфенова ВВ, Белькова НЛ, Суханова ЕВ, Гладка ГВ, Таширев АБ. Аутэкология, таксономия и стратегия выживания в экстремальных условиях антарктических микроорганизмов. Фундаментальные исследования. 2014; 11(9):1954-1959.
  8. Govorukha V, Tashyrev O. Thermodynamic prognosis for assessing the role of Fe(III)-reducing bacteria in biogeochemical cycles of iron and carbon. Ecological Engineering and Environment Protection. 2014; 3-4:45-54.

Ukrainian

  1. Романовская ВА, ПарфеноваВВ, БельковаНЛ, СухановаЕВ, Гладка ГВ, Таширева АА. Филогенетический анализ бактерий экстремальных екосистем. Мікробіол. журнал. 2014;76(3):3-10.
  2. Варбанець ЛД, Мацелюх ОВ, Авдіюк КВ, Гудзенко ОВ, Нідялкова НА, Романовська ВО, Таширев ОБ. Гідролітична активність мікроорганізмів, виділених з екосистем узбережжя Мертвого моря. Мікробіол. журнал. 2014; 76(1):16-20.
  3. Таширев АБ, Суслова ОС, Рокитко ПВ, Бондарь КМ, Покалюк ВВ. Экофизиологическая характеристика азотфиксирующих бактерий карстовых полостей Мушкарова Яма и Куйбышевская. Наукові записки Тернопільського національного педагогічного університету ім. В.Гнатюка. Серія: Біологія. 2014;60(3):169-174.
  4. Таширев АБ, Суслова ОС, Рокитко ПВ, Олексенко АА, Бондарь КМ. Устойчивость азотфиксирующих бактерий карстовых полостей к экстремальным факторам. Biotechnologia Acta. 2014; 5: 43-49.
  5. Tashyrev OB, Pidgorskyi VS, Miguel Naranjo Toro, Miguel Gualoto, Gladka GV, Tashyreva HO, Rokitko PV, Romanovskaya VA. Autecology of microorganisms of typical Ecuador biotopes. Мікробіол. журнал. 2014; 76(6):3-10.
Monographs
  1. Singh N, Devi A, Bishnoi M, Jaryal R, Dahiya A, Tashyrev O, Govorukha V.  Overview of the Process of Enzymatic Transformation of Biomass.  Elements of Bioeconomy Edited by Krzysztof Biernat. – IntechOpen Limited, London, United Kingdom. 2019. DOI: http://dx.doi.org/10.5772/intechopen.85036 (розділ книги)
  2. Таширев О.Б. Розробка біотехнології отримання молекулярного водню при максимальній деструкції харчових відходів /О.Б.Таширев, В.М.Говоруха, О.А.Гаврилюк, І.Б.Сіома та ін. // Фундаментальні аспекти відновлювально-водневої енергетики і паливно-комірчаних технологій / за загальною редакцією Ю.М. Солоніна. – К.: «КІМ». 2018. 260 с. http://www.materials.kiev.ua/Hydrogen/Book_printVer.pdf (розділ книги)
Patents
  1. Pat. 10809 UA, MPK5 СО2 F 1/28, 3/34; G21 F9/18.– Publ. 26.02.1999, Bull. No. 1. Method of purification of aqueous solutions from metal ions by biomass of microorganisms. Tashirev OB, Shevel VM.
  2. Pat. 34894 A Ukraine, MKY6 C 02 F 3/34. -Published 15.03.2001, Bull. No. 2. "Rodoil" preparation for biological cleaning of soil and water from contamination by oil and oil products. Dulgerov OM, Nogina TM, Pidgorsky VS, Havrylenko MN, Dumanska TU.
  3. Pat. 50585А UA, IPC 7С22В3/00, С02F1/00. Publ. 15.10.2002, Bull. No. 7. The method of extraction of a wide range of metals from aqueous solutions by the biomass of mixed microbial communities. Tashirev OB, Tashireva GO.
Cooperation

The University of Southern Mississippi, Hattiesburg, USA (Університет Південного Міссісіпі, Хаттісбург, США)

The Oklahoma State University, Stillwater, USA (Державний Університет Оклахома, Стіллвотер, США)

Guru Jambheshwar University of Science and Technology, Hisar, India (Гуру Джамбхешвар Університет Науки і Технології, м. Хісар, Індія)

Institute of Microbiology "Stefan Angelov" (Sofia, Bulgaria)

Gas Institute of the National Academy of Sciences of Ukraine

Limnological Institute of the Siberian Branch of the Russian Academy of Sciences

National Aviation University

Antwerp Maritime Academy, Belgium (Морська Академія Антверпена, Бельгія)

LLC "International Center of Gas Technologies"

Employees of the department

Oleksandr Tashirev

Head of the department, DSc., prof., specialist in the field of thermodynamic prediction of the interaction of microorganisms with a wide range of xenobiotics (metals, radionuclides, synthetic organic compounds, etc.), is engaged in the development of environmental protection and energy biotechnologies for cleaning ecosystems from pollution and obtaining energy carriers (hydrogen, methane , solid fuel), as well as metal concentrate, purified water.

ResearchGate

Scopus

Orcid

Google Scholar

Vira Hovorukha

researcher, Ph.D., specialist in the field of environmental protection and energy biotechnologies, is engaged in researching the patterns of interaction of microorganisms with toxic metals based on thermodynamic calculations and optimizing the process of destruction of environmentally hazardous mixed food waste and obtaining an environmentally clean energy carrier - molecular hydrogen.

ResearchGate

Scopus

Orcid

Google Scholar

Halyna Hladka

researcher, Ph.D., a specialist in the field of taxonomy and autecology of microorganisms, is engaged in researching the taxonomic diversity of microorganisms in extreme regions, as well as their autecology, in particular, resistance to extreme factors (UV radiation, dehydration, hypersalinity, temperature).

Scopus

Orcid

Google Scholar

Olesia Havryliuk

PhD student, leading engineer, specialist in environmental biotechnologies and environmental bioremediation. She is engaged in the study of the ability of phytobacterial communities to interact with compounds of toxic copper and remove it from contaminated soils. Researches copper-resistant strains of microorganisms of natural and man-made ecosystems and molecular-biological mechanisms of their resistance to copper compounds.

ResearchGate

Scopus

Orcid

Google Scholar

Iryna Bida

Leading engineer, the field of work is the fermentation of solid food waste with the production of hydrogen and the extraction of toxic metals from solutions.

Orcid

Google Scholar

Yanina Danko

Engineer, field of work - fermentation of liquid food waste with methane production.

Oleksandra Shablii

Engineer, field of work - distribution of microorganisms in natural ecosystems and their resistance to toxic compounds.

Oleksandra Kliara

Engineer, direction of work - treatment of metal-containing wastewater.

Awards
  •   the award of the National Academy of Sciences of Ukraine for the supervision of a scientific generation was awarded to: Head of Department, doctor of technical sciences, prof. Tashirev O.B.
  •   the award of the National Academy of Sciences of Ukraine for young scientists "Talent, inspiration, work" was awarded to: Govorukha V.M.
  •   the scholarship of the President of Ukraine was awarded to: Govorukha V.M.
  •   the National Academy of Sciences of Ukraine scholarship for young scientists was awarded to: M.Sc., Candidate of Science V.M. Govorukha; graduate student, leading engineer Gavrilyuk O.A.