Відділ мікробіологічних процесів на твердих поверхнях - ІМВ НАН України 🇺🇦

Head of Department, Doctor of Biological Sciences,

Professor Ivan Kurdysh 

CONTACTS:

 Україна, 03143, Київ,

вул. Академіка Заболотного, 154;

phone: +38 (044) 294-69-68

E-mail: ivan.kurdish2016@gmail.com 

History

In 1988, at the Institute of Microbiology and Virology named after D.K. Zabolotny of the National Academy of Sciences of Ukraine, a laboratory of microbiological processes on solid surfaces was created, which in 1997 was reorganized into a department of the same name. The founder and head of this unit is Doctor of Biological Sciences, Professor I.K. Kurdysh.

The main direction of the scientific activity of the department is the study of the interaction of methanotrophic, nitrogen-fixing, phosphate-mobilizing and other microorganisms with solid surfaces, including nanomaterials of various nature, as well as with components of agroecosystems.

Scientists of the department paid considerable attention to the development of biotechnology to reduce methane emissions from the produced spaces of coal mines, from which up to 80% of the total amount of methane enters the coal mining area. This creates a danger to the work of miners.

Scientific work

Physiological and biochemical properties of methanotrophic bacteria, patterns of their interaction with solid surfaces, including mine rocks, were investigated. Ionogenic determinants of the surface of bacteria, which determine the electrosurface properties of cells and their adhesion to solid materials, have been determined. The important role of hydrophobicity of bacteria in their interaction with solid materials has been established, the patterns of adhesion of pure and mixed cultures of methanotrophs to rocks and other materials have been determined, and approaches to the immobilization of these microorganisms on the surface of these rocks have been developed.

        In cooperation with the employees of the Institute of Geotechnical Mechanics named after M.S. Polyakov of the National Academy of Sciences of Ukraine developed the biotechnology of obtaining biomass of methanotrophic bacteria on mine methane and the biotechnology of reducing methane emission from the produced spaces of coal mines by these bacteria immobilized on the surface of rocks. The application of this biotechnology at a number of Donbas mines allowed to reduce methane emissions at coal mining sites by 47-60%.                          

        Employees of the department found that the interaction of microorganisms with solid surfaces, including nanomaterials of various origins, had a noticeable effect on the physiological and biochemical activity of populations: it increased their growth and enzymatic activity, the synthesis of biologically active substances, etc. One of the factors determining these processes is the increase in oxygen mass transfer when nanomaterials are introduced into the liquid medium for cultivating microorganisms.

        If nanoparticles are present in the medium, cells enter into contact interaction with them. As a result, nanoparticles are absorbed on the surface of bacteria. Such interaction is accompanied by a significant increase in cell resistance to adverse environmental factors, including supraoptimal temperatures. The results of the research became the basis of the biotechnology of increasing the yield of viable cells in dry bacterial preparations during their spray drying. This biotechnology was implemented at the Kyiv Medical Products Factory of the "Ukrbiohimpreparat".

         We selected highly active strains of nitrogen-fixing bacteria Azotobacter vinelandii  IMV B-7076 and phosphate-mobilizing bacteria Inacillus subtilis IMV B-7023. The latter, along with the ability to mobilize phosphate from organic and sparingly soluble inorganic compounds, are characterized by high antagonistic activity against phytopathogenic bacteria and fungi. These strains are able to accumulate a number of biologically active substances in culture media: organic acids, amino acids, compounds of phenolic nature, phytohormones.

     The physiological and biochemical properties of the selected strains were studied Azotobacter vinelandii IMV B-7076 and Вacillus subtilis ІМВ В-7023, особливості їх   взаємодії   з наночастками   природних   мінералів.   Встановлено, що завдяки взаємодії бактерій з частками глинистих мінералів значно підвищується життєздатність клітин за впливу негативних факторів середовища. Отримані результати стали науковою основою розробки біотехнології створення гранульованого,  сипкого та нанокомпозитного  комплексного бактеріального препарату Азогран. Він є високостабільним за тривалого зберігання.

        The primary stages of the interaction of bacteria-components of a complex bacterial preparation with plants were studied (chemotaxis properties of bacteria, regularities of their adhesion to roots, etc.). It was established that the introduction of nanoparticles into the suspension of bacteria is accompanied by an increase in their energy potential, chaotic mobility and a decrease in chemotaxis. The latter is probably due to the blocking of chemotaxis receptors on the surface of cells by these particles. In a mixed culture Azotobacter vinelandii and Вacillus subtilis chemotaxis of bacilli is significantly reduced. It was established that this is due to the blocking of chemotaxis receptors on their surface by the exopolysaccharide complex of Azotobacter.  

         Cells Azotobacter vinelandii and Inacillus subtilis weakly adhere to solid materials. However, they are attached to the surface of plant roots in significant quantities. Their adhesive properties are to a certain extent determined by the mobility of cells, the features of their surface, and the methods of introducing bacteria into the agroecosystem. The presence of lectins on the surface of bacilli, their carbohydrate specificity and a significant role in adhesion have been established Вacillus subtilis to plant roots.

       It has been shown that the complex bacterial preparation Azogran is capable of significantly inhibiting the spread of phytopathogens and phytophages (whitefly, Colorado potato beetle, etc.) in agroecosystems. Its use in agro-ecosystems significantly stimulates the growth of lawn grass, decorative and flower plants, increases the yield of technical, grain and vegetable crops by 18-37%.

      The preparation Azogran is registered in Ukraine. Licenses for its production have been granted to 8 enterprises of Ukraine: NVF "Rovial", SE "Enzym", LLC "Agrarian biotechnology", "Agro-Admiral", PE "Biochem-Service", "Bio Dobrivo", "Biotech Active", "Karat" -Bio", as well as the American company "Locus Solution LLC".

     The development of the scientific basis for the creation of new generation microbial preparations was awarded the prize of the National Academy of Sciences of Ukraine named after D.K. Zabolotny (2005). I.K. Kurdysh is the co-author of the series of works "Biologically active substances of microbial synthesis in the latest biotechnologies and modern agricultural production", which was awarded the State Prize of Ukraine in the field of science and technology in 2018.

      Employees of the department have published 6 monographs and more than 360 scientific works, including 35 articles in foreign journals and 16 patents. Employees of the department defended 8 PhD theses.

       The department's achievements are due to the friendly work of its team. A significant contribution to this belongs to Doctor of Biological Sciences N.F. Kigel, PhD in Technical Sciences A.S. Gordienko, PhDs in Biological Sciences, A.O. Roy, O.G. Kistenyu, L.V. Tytova, N.V. Chuyko, L.V. Bulavenko, L.S. Tserkovnyak, D.I. Dyrenku, A.Yu. Chobotaryov, I.O. Skorokhod, leading engineer Z.T. Bega and other colleagues.

Monographs
 Microbiological oxidation of methane in coal mines.Киев: Наукова думка,        –   1991. -145 с.Мякенький В.И., Курдиш И.К.
 Гранулированные мікроб-ные препараты для растениеводства: наука и практика.Киев: РІВЦ.    -2001. -141 с.Курдиш И.К.
 Медицинская химия и Клиническое  применение диоксида кремния.Киев: Наукова думка. -2003. -414 с.Колект. моногр. под ред.А.А.Чуйко.
 Інтродукція мікроорганізмів у агроекосистеми.Київ: Наукова думка. -2010. -253с.Курдиш І.К.
 Наноразмерные системы и наноматериалы: исследования в Украине.Киев, Академпериодика,  -2014. 766с.Кол.монографія під ред. А.Г.Наумовця.
 Nanobiotechnology in Bioformulations.Munchen, 2019. Springer.      -491 c.  Колективна монографія    під ред.  Ram Prasad et al.
Publications
1.Influence of Silica Nanoparticles on Antioxidant Potential of Bacillus subtilis IMV B-7023Nanoscale Research Letters.-2016.11:139. doi: 10.1186/s11671-016-1348-2.Iryna O.Skorochod, Alla O.Roy, Ivan K.Kurdich
2.Influence of Physical and Chemi-cal Factors on the Accumulation of Phenolic Compounds Nitrogen-Fixing Bacteria Azotobacter vinelandii IMV B-7076.Acta Velit.-2016.-V.3,№1.-P.49-55.Anton Ocheretyanko, Alla Roy, Iryna Skorocod, Ivan Kurdish
3.Specificity оf adhesion of Bacillus subtilis IMV B-7023 to abiotic and biotic surfaces.Journal of Microbiology, Biotechnology and Food Science.  -2016. -6,№1.           -Р.652-654.   doi:10.15414/jmbfs.2016.6.1. 652-654.                Ivan Kurdish, Alla Roy, Dmytro Dyrenko, Kateryna Hetman, Emma Kovalenko
4.Accumulation of phytohormones by soil bacteria Azotobacter vinelandii and Bacillus subtilis under the influence of Nanomaterials.Journal of Microbiology, Biotechnology and Food Science.-2017.18.7.3 -P.271-274.  doi: 10.15414/jmbfs. 2017.18.7.3. 271-274.  Andrii Chobotarov, Mykola Volkogon, Lesya Voytenko, Ivan Kurdish.
5.Взаємодія бактерій з твердими матеріалами та наноматеріалами як основа нових біотехнологій.Мікробіол. журн. 2018.  – 50, №3     -С.15-28. doi:https://doi.org/10.15407/ microbiolj80.03.015.Курдиш І.К.
6.Natural Mineral Nanoparticles and Some Cations Effect on Growth-Regulating and Superoxide Dismutase Activity of Azotobacter vinelandiiiIMV B-7076. Місrobiol. Z.-2020.-82.№2. -С.22-29.Kurdish I.K., Chobotarov A.Yu  
7.Antagonistic activity of Azotobаcter vinelandii IMV  microorganisms.B-7076 against phytopathogenic Mikrobiol. Z. 2020. -82.№5.                 -Р.21-29.  N.V. Chuiko, A. Yu. Chobotarov, Ya. I. Savchuk,  I. M. Kurchenko,  I. K. Kurdish.
8.Influence of a nanocomposite biological product of Azogran on barley seeds exposed to oxidative stress.Scientific Light. 2020, № 36. -P.10-14.  Skorochod I.A., Ulziijargal E., Кurdish I.K., Gorgo Yu.P.
9.Content of organic acids in the cultural medium of Bacillus subtilis IMV B-7023 at cultivation with different sources of the phosphorus nutrient. Journal of Microbiology, Biotechnology and Food Science.-2020.10.1. -P.73-77. doi:10.15414/jmbfs.2020.10.1.73-77.Iryna Skotochod, Alla Roy, Ivan Kurdish, Ulzijargal Erdenetsogt.
10.Antioxidant action of a nanocomposite biological product Azogran on seeds development of different varieties of barley.International Journal of Scientific and Research Publications, Volume 10, 4, 2020. Р.154-158.  E.Ulziijargal, I.O.Skorochod , I.K.Kurdish ,  A.A.Roy , Yu.P.Gorgo.
11.Effect of nanoparticles of natural minerals,iron and mangan compounds ,on the growth and superoxide dismutase activity of Bacillus subtilis IMV B-7023.Journal of Microbiology, Biotechnology and Food Science.-2020.10.1. -P.130-133.Ivan Kurdish, Andrii Chobotarov, Roman Gritsay.      
12.      Biologically active composite based on fumed silicia and Amoectochilus formasanes Hayata extract  Chamical Paper (2021) 75:       2575-2583 https//doi.org/10.1007/s11696-021-01513-1.Roman Іvannikov, Iryna Laguta, Oksana Stavinskaya, P.O. Kuzema, Iryna Skorochod, Alla Roy,  Ivan Kurdish
13.Efficiency of the Complex Bacterial Preparation Azogran Application in Protectting Potatoes from the Colorado Potato Beetle  Depending on the Stage of its Development.Mikrobiol. Z. 2021. 83. №1.   -С. 3-11. doi:https://doi.org/10.15407/ microbiolj83.01.003Kurdish I.K., Roy A.A., Skorochod I.A.
14.Natural and synthetic nanomaterials in microbial biotechnologies  for crop production.Mikrobiol. Z.  2021. 83. №3.    –С.81-91. doi:https://doi.org/10.15407/ microbiolj83.03.081.  Kurdish I.K.