FEATURES OF ANTIBIOTICRESISTANT STRAINS OF THE ASSOCIATION ESCHERICHIA COLI-ENTEROCOCCUS FAECALIS OF THE UROGENITAL TRACT OF PREGNANT WOMAN
Lusta M, Voronkova O.
Oles Honchar Dnipro National University
e-mail: maksimlusta12@gmail.com
Uncontrolled use of antibiotics makes a reservoir of resistance determinants among pathogenic and opportunistic bacteria. According to the results of clinical studies, 88% of strains from the urine of pregnant women are enterobacteria, among which 80% – E. coli, and 15% – E. faecalis. It was found that E. coli strains are 65% resistant to ampicillin, 20% – to amoxicillin/clavulanic acid, 30% – to trimethoprim. Tolerance to cephalosporins is 25%, to nitrofurantoin – 10%, fosfomycin – 7%. Resistance of E. faecalis to tetracycline is 95%, to co-trimoxazole – 20%. 96% of enterococci are sensitive to ampicillin, nitrofurantoin, carbapenems and ciprofloxacin.
The antibiotic resistance of urostrains of the association of E. coli – E. faecalis of pregnant women in comparison with the corresponding indicators of monostrains of these bacteria was studied.
Bacteriological method of sectoral culture of urine on nutrient media was used and identification of bacterial strains to the species was performed. The susceptibility of bacterial strains to antibiotics was determined by disk-diffusion method, the interpretation of results – according to current EUCAST recommendations. Statistical processing was performed in MS Excel.
It was found that among 100 strains of E. coli, 45 urostrains (45%) are resistant to at least one antibiotic, of which 13 (28%) cultures – to fosfomycin, 8 (17%) – to cefuroxime, 12 (27%) – to cephalexin, 2 (4%) to nitrofurantoin, 12 (27%) to norfloxacin and 36 (80%) to trimethoprim. Among 100 urostrains of E. faecalis, 55 isolates (55%) are resistant to at least one antibiotic, of which 7 (13%) strains – to ampicillin, 13 (24%) – to norfloxacin, 49 (89%) – to trimethoprim, 4 (7%) – to nitrofurantoin.
Among the 50 associated E. coli urostrains, 26 isolates (52%) are resistant to at least one antibiotic, against 45 strains (45%), of which 11 strains (42%) – to fosfomycin, 4 (15%) – to cefuroxime, 5 (19%) to cephalexin, 6 (23%) to norfloxacin and 19 (73%) to trimethoprim. Among the 50 associated urostrains of E. faecalis, 28 isolates (56%) are resistant to at least one antibiotic, against 55 strains (55%), of which 3 (6%) strains – to ampicillin, 2 (4%) – to norfloxacin, 26 (52%) – to trimethoprim, 3 (6%) – to nitrofurantoin.
It was found that the number of resistant strains of E. coli in the association is 7% higher than in strains. Resistance to fosfomycin increased from 28% (single strain) to 42% (associated strain). The number of resistant enterococci did not differ in both monocultures and associations.