Active producers were selected as a result of studies on the ability of exoproteases of supernatants of culture liquids of bacteria isolated from bottom sediments of the Black Sea to hydrolyze fibrin, fibrinogen, and collagen. It was shown that partially purified enzyme preparations of Bacillus subtilis 248 and Bacillus subtilis 231 differed in physicochemical properties. Thus, the fibrin-(ogen)olytic activity of B. subtilis 248 has a pH optimum of 9.0, and the thermooptimum of fibrinogenolytic activity is 4-20 °C, while that of fibrinolytic activity is 15-20 °C. The partially purified enzyme preparation B. subtilis 231 has two pH optimums of fibrin(ogen)olytic activity – 7.0 and 11.0, and the thermooptimum – 37 °C. It was established that Bacillus subtilis 248 and Bacillus subtilis 231 may be promising for further scientific research as producers of proteases with α/β/γ-fibrinogenase and α/β-fibrinase activity.
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