Aim To study the susceptibility of isolates obtained from the oral cavities of individuals with inflammatory periodontal diseases and maxillofacial pathology to bacteriophages. Material and methods Biological specimens were obtained from the Dental Medical Center and University Clinic of O.O. Bogomolets National Medical University and the National Specialized Children’s Hospital OKHMATDYT. Biomaterial was collected from the wound surface using a sterile FLmedical transport system (Italy). Clinical isolates used in the studies included Escherichia coli, Klebsiella spp., Pseudomonas spp., and Staphylococcus aureus. We assessed the susceptibility of microorganisms to bacteriophages by administering 0.01 ml of bacteriophage culture to a bacterial culture. A suspension (inoculum) was prepared from a 24-hour culture of microorganisms in sterile saline and then Mueller Hinton agar was inoculated with it. The results were recorded 24 hours post-incubation in a thermostat at 37°C. The study used bacteriophages from the bacterial test cultures Klebsiella pneumoniae DSM30104, Staphylococcus aureus DSM 799, Escherichia coli DSM 1103, and Pseudomonas aeruginosa DSM 50071, provided by NeoProBioCare Ukraine Ltd. Results The susceptibility rates of isolates to phages were 40% for Klebsiella spp, 73.3% for S. aureus, 50.0% for E. coli, and 20.0% for Pseudomonas spp. Prior research confirms the effectiveness of bacteriophages in oral diseases. This method may be beneficial in controlling opportunistic microorganisms and maintaining the oral microbiota balance. Conclusions The application of bacteriophages for inflammatory oral diseases appears promising and warrants further research. Clinical trials and additional scientific investigations are necessary to confirm its safety and effectiveness.
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