Department of Virus Reproduction

Complex structural and functional study of adenovirus capsid proteins (hexon, fiber). Studies of the physico-chemical properties of human adenovirus type 1 proteins were conducted and new fundamental knowledge was obtained about the features of the localization of adenovirus proteins in an infected cell, several new antigenic determinants of the hexon and fiber of human adenovirus type 1 were discovered, their different nature was revealed (conformational or linear) as well as the orientation, which depends on the spatial organization of proteins, the localization of some epitopes was determined using chemically synthesized antigenically active peptides imitating them.

• Significant regularities of the structural and functional organization of the hexon of adenoviruses were revealed on the basis of a comparative analysis of antigenic specificity and the primary structure of proteins of different adenoviruses taxonomically distant from each other. The concept of immunoinactivation (neutralization of infectivity) of adenoviruses was developed and a mathematical model of this process was created for the first time, which determines the contribution of antibodies to several antigenic determinants of hexon and fiber, as well as interferon and complement.
• Within the framework of the INTAS 94-292 grant, work on the synthesis and processing of viral polypeptides of adenovirus 4 and 4a was carried out. Mapping of the antigenic determinants of the adenovirus hexon and fiber was done. The molecular characteristics of cellular receptors were determined using fragments of the fiber and hexon genes.
• Study of features of adenovirus genome expression during productive infection in various cellular systems, as well as under the influence of macromolecule synthesis inhibitors and substances with antiviral activity promising for chemotherapy. Directed search and research of the mechanisms of action of anti-adenoviral substances in the department was started in 1979 together with Dr. Chernetsky V.P. and his students Alekseyeva I.V. and Shalamay A.S. (Institute of Molecular Biology and Genetics of the National Academy of Sciences of Ukraine). The influence on the reproduction of human adenoviruses of the original structural analogue of cytidine (2-β-D-ribofuranosyl-5-amino-1,2,4-triazine-3(2H)-OH) – 6-azacytidine, was investigated in the most detailed manner. Due to its low cytotoxicity and high anti-adenoviral effect, the drug is patented as an antiviral. It has been established that its antiviral activity is associated with inhibition of the synthesis of viral DNA and proteins. A number of 6-azacytidine derivatives were studied and compounds with lower toxicity and better solubility were identified.
• Anti-adenoviral activity was found in 6-azauridine, a structural analogue of uridine synthesized by Ukrainian chemists. The activity of cytosinarabinoside derivative – cyclocytidine and ribavirin’s structural analogue – ribamidil, synthesized by the corresponding member of the Academy of Sciences of Latvia Ladakh M.Yu. was also studied. It was shown that ribamidil affects the formation of a functionally complete hexon and the maturation of adenovirus virion.
• The antiviral activity of proteolysis inhibitors – amben and ε-aminocaproic acid was studied. The target of their action is a violation of the morphogenesis of mature infectious virions, which is caused by a disruption of the processing of the precursor of the internal polypeptide (VII).
• Within the framework of the grant INTAS 97-31528 together with the employees of the A.V. Bogatsky Odesa Institute of Physics and Chemistry of the National Academy of Sciences of Ukraine (V.E. Kuzmin, A.G. Artemenko) the antiviral properties of macrocyclic pyridinophanes were investigated using 3D and 4D QSAR modeling, which allows establishing the relationship between the structure and activity of the substance, as well as to predict the effectiveness of compounds against viruses. With the use of QSAR and simplex representation, active structures were discovered and new analogues were synthesized for which the results of antiviral activity were obtained.
• From the aqueous extract from Artemia cysts, the employees of the Institute of Southern Seas of the National Academy of Sciences of Ukraine (I.I. Rudneva and V.G. Shaida) were obtained extracts that were patented as an antiviral agent with anti-adenoviral and anti-herpetic activities.

• The first Ukrainian immunoenzymatic test system for the diagnosis of diseases caused by the Epstein-Barr virus has been developed. The antigen was obtained, its specificity was confirmed, and panels of positive and negative sera were created. The conditions for enzyme-linked immunosorbent assay were optimized and the specificity, sensitivity, and compliance parameters of the “ІФА-АтВЕБ-стрип” test systems were characterized.
• Within the framework of the All-State (national) space program of Ukraine for 2003-2007, the influence of conditions of simulated microgravity on human adenovirus and the cell-virus system was studied. The perspective of using adenovirus as a model for studying the influence of microgravity was shown. It was established that the intact human adenovirus is capable of maintaining infectivity for a long time and reproducing in conditions of simulated gravity.
• Within the framework of grant INTAS 011-2382, the impact of the Epstein-Barr virus on signal transduction processes in malignant B-cells transformed by the virus was investigated. Experimental virus-cell systems were developed, in which the influence of viral proteins on the process of CD-95-mediated apoptosis in the conditions of drug-induced apoptosis was studied. Data were obtained on the different degree of influence of viral infection on the sensitivity of BL-41 and DG-75 cells to etoposide and doxorubicin, which are antitumor agents. This may be caused by different mechanisms of their action, which in one way or another limit the replicative mechanism of EBV.

• A model of mixed adeno-herpetic infection was created on a population of suspension lymphoblastoid and monolayer transplantable human and animal cells, and the effect of antiviral drugs in the developed models was investigated. Model systems of co-infected cell cultures were obtained for the first time, the reproduction of viruses in them was proven, and a detailed analysis of the structural and functional virus-cell interaction was carried out. The discovered anomalous effect of antiviral substances in the case of co-infection of cells with two viruses indicates the need to use such methodological techniques in the study of promising antiviral substances. The proposed approach of research of promising antiviral compounds on the model of mixed viral infection can take place when creating new and studying the activity of already existing chemotherapeutic drugs, which may help prevent the development of viral resistance to drugs.
• Developed and published: educational and methodological manual (Dzyublik I.V., Dyachenko N.S., Rybalko S.D., Shcherbinska A.M., Nosach L.M., Voronenko S.G., Porokhnytskyi V.G. The guide to chemotherapy of viral infections for doctors/ edited by Dzyublik I.V.-K., 2004.-176 p.); methodological recommendations for preclinical studies of the specific antiviral effect of drugs in cell culture on the model of adenoviruses (Shcherbinska A.M., Dyachenko N.S., Rybalko S.L., Nosach L.N., Dyadyun S.T., Vrynchanu N. O. Study of the antiviral effect of potential medicinal products//Methodological recommendations of the Ministry of Health, State Pharmacological Center. – 2000. – 40 p.); methodological recommendations (Nosach L.M., Povnytsia O.Yu. Preclinical study of the specific antiviral effect of drugs in cell culture on the adenovirus model. Methodical recommendations // Herald of Pharmacology and Pharmacy. – 2007, No. 9. – P. 52-64) .
• Together with BIOPHARMA PLAZMA LLC, the release of specific immunoglobulins against TORCH infections (cytomegalovirus, herpes 1, 2 and 6 types, Epstein-Barr virus and chlamydia) has been established.

• For diagnostic purposes, immunosensors were created for the detection of specific antibodies to the Epstein-Barr virus, adenovirus, and herpes simplex virus in the blood sera of patients using the Plasmon-6 optoelectronic device.
• The biological activity of newly synthesized fluorinated compounds of nucleoside and nucleotide nature was investigated. Substances based on a chloro-substituted furan (5-membered) ring showed anti-adenoviral activity, the titer of the synthesized de novo virus decreased by >98%. Effective inhibition of HSV-1 reproduction by triazole derivatives was shown in the in vitro and in vivo systems (efficiency index is 100%). Some fluorinated compounds had both polyvalent and monospecific effects against human adenoviruses and herpesviruses and can be prototypes for the creation of new etiotropic drugs.
• The effect of composite drugs, which combine the properties of biologically active materials and nanoparticles (compound G29 – fluorinated 1,2,3-triazine and TiO₂ nanoparticles; official drugs Ganciclovir and Neoflazid) was investigated. When using composites, an increase in the antiviral effect was found at low concentrations of drugs.
• The cytotoxic, virucidal and antiviral effects of nanoparticles of zinc, titanium, cerium and lanthanum oxides were investigated. Their insignificant cytotoxic effect, high virulicidal effect and photoactivation abilities are shown.
• Using an iterative de novo approach, a molecule with significant affinity to four SARS-CoV-2 variants – Wuhan, Omicron, Delta and Cluster 5 was designed (specifically to their receptor-binding domain of S-glycoprotein, in particular to the site that directly involved in the recognition of human ACE2). The developed molecule has a high potential to be an effective drug against SARS-CoV-2. Further studies are planned to be conducted in vitro and in vivo.

The main areas of research of the department in 2024

• The study of optimal schemes for the use of various chemical, natural substances (classes of compounds) and physical (UV irradiation, ozonation) methods of influencing the reproduction of the influenza A virus, adeno- and herpesviruses. Prospects for their combined use.
• Determination of antiviral activity of compounds and probable targets of their action by in silico methods (molecular dynamics sumulation and molecular docking).

• 2020-2024 Scientific research project of the National Academy of Sciences of Ukraine. №0115U004127 “Regularities of influence of chemical, natural and physical virus-inactivators on biological processes in the virus-cell system”.
• 2023-2024 Scientific research project “Study of the antiviral activity of synthesized compounds in vitro and in vivo against influenza virus, adenovirus and SARS-CoV-2 model systems” within the framework of the National Academy of Sciences of Ukraine project “Prediction of bioactivity and creation of modern approaches to the synthesis of heterocyclic compounds for the needs of the pharmaceutical industry”, (the program “Support of state-priority scientific research and technical (experimental) developments”).
• 2023-2025 NATO International Project, SPS MYP G6085 “Establishment of a Strategy for the Assessment and Restoration of War-Damaged Aquatic Ecosystems”             

• IX International Electronic Conference on Medicinal Chemistry, Department of Organic Chemistry, University of Mons-UMONS, November 1-30, 2023 Belgium;
• X International Conference „Bioresources and Viruses“ September 11-13, 2023, Kyiv, Ukraine;
• 8th International Materials Science Conference HighMatTech-2023
  October 2-6, 2023 Kyiv, Ukraine;
• Ukrainian Conference with International Participation “Chemistry, Physics and Technology of surface” October 11-12, 2023 Kyiv Ukraine:
• The International Scientific and Practical Conference “Modern aspects of microbiology, virology, and biotechnology in wartime and post-war period” November 15-16, 2023 Kyiv, Ukraine;
• International research and practice conference “Nanotechnology and Nanomaterials” August 25-27, 2022, Lviv, Ukraine;
• 50^th International School and Conference on the physics of semiconductors “Jaszowiec 2022” June 4-10, 2022 Szczyrk, Poland;
• 2^nd International Research and Practice Conference «Nanoobjects & Nanostructuring» (N&N-2022). September 26-28, 2022 Lviv, Ukraine;
• Ukrainian Conference «Chemistry, physics and technology of surface». October 19-20, 2022 Kyiv, Ukraine;
• The 26th International Electronic Conference on Synthetic Organic Chemistry. November 15-30, 2022, Basel, Switzerland;
• VII International Electronic Conference on Medicinal Chemistry. University of Mons-UMONS, November 1-30, 2021 Belgium;
• 7^th International  Materials Science Conference HighMatTech-2021, Kyiv, Ukraine;
• Conference “Safety and regulation of the engineering nanomaterials”, University LUSOFONA, 2020, Lisbon;
• International Conference on Antiviral Research (ICAR), May 12-15 2019 Baltimore, Maryland, USA;
• 5th International Electronic Conference of Medical Chemistry 1-30 November 2019, Belgium;
• Іnternational biological, agricultural & life science congress November 7-8, 2019, Lviv, Ukraine;
• IX International Conference “Bioresources and viruses”, September 9-11, 2019, Kyiv, Ukraine;
• 3^rd International Conference “SmartBio”, 2-4 May 2019, Kaunas, Lithuania;
• Ukrainian Conference with international Participation “Chemistry , physics and technology of surface” and Workshop “Metal-based biocompatible nanoparticles: synthes and applications”, Kyiv, 2019, Ukraine;
• 6^th Ukrainian congress for cell biology with international representation, 18-21 June 2019, Yaremche, Ukraine;
• Young Scientists conference “Youth and modern problems of microbiology and virology” 12014 November 2019, Kyiv, Ukraine;
• З’їзди товариства мікробіологів України 2000-2019 рр., Україна;

2024

1. Yavorovsky O.P., Riabovol V.M., Zinchenko T.O., Zahornyi M.M., Ragulya A.V., Tyschenko N.I., Povnitsa O.Yu., Artiukh L.O., Zahorodnia S.D., Ostapiv D.D. Comparative toxicological-hygienic assessment, structural-morphological, physicochemical characteristics, and virucidal properties of new nanopowder materials TiO2 and TiO2@Ag. Medicni perspektivi. 2024; 29(1):180-192.https://doi.org/10.26641/2307-0404.2024.1.301212   
2. Zaremba, P., Zaremba, A., Siry, S., Zahorodnia, S. (2024). Antiviral Activity of Low-Molecular-Weight Fluorinated Compounds Against Influenza A (H1N1) Virus. Mikrobiolohichnyi Zhurnal, 86(2), 51-64. https://doi.org/10.15407/microbiolj86.02.051
3. Rybalchenko, N., Hnatiuk Т., Artiukh, L., Naumenko К., Zaremba, P., Demchenko, V., Kokhtych, L., Iurzhenko, M., Rybalchenko, T., Оvsankina V., Dolgoshey, V., Sytnyk, I., & Marynin, A. (2024). Antimicrobial and Antiviral Activity of Nanocomposites Based on Polyelectrolyte Complexes with Silver Nanoparticles. Mikrobiolohichnyi Zhurnal, 86(2), 36-50. https://doi.org/10.15407/microbiolj86.02.036
4. Tkachuk, N., & Zelena, L. (2024). Microbiological indicators of the biofilms microparticles of quartz sand and polypropylene after short-term exposure in soil. Biofouling, 1–12. https://doi.org/10.1080/08927014.2024.2406340
5. Balko, O. B., Zelena, L. B., Balko, O. I., Bobyr, N. A., Voitsekhovsky, V. G., & Avdeeva, L. V. (2024). Phenotypic and Genotypic Criteria for the Screening of Highly Active S-Type Pyocins Pseudomonas aeruginosa Producers. Mikrobiolohichnyi Zhurnal, 86(1), 39-50. DOI: https://doi.org/10.15407/microbiolj86.01.039
6. Pidkurhanna, O.H., Zelena, L.B. & Shulha, S.M. Creation of Heme and Hemoglobin Producers Based on Microorganisms. Cytol. Genet. 58, 300–311 (2024). https://doi.org/10.3103/S009545272404008X
7. Tkachuk, N.; Zelena, L. Mechanisms of Biodeterioration of Structural Materials by Streptomyces spp.: A Review. Eng. Proc. 2024, 67, 12. https://doi.org/10.3390/engproc2024067012
8. Демченко, В. Л., Загородня, С. Д., Рибальченко, Н. П., Артюх, Л. О., Заремба, П. Ю., Юрженко, М. В., Ситник, І. О., Овсянкіна, В. О., Неймаш, В. Б. (2024). Полімерні гелеві матеріали на основі полівінілового спирту і наночастинок срібла. [Polymer gel materials based on polyvinyl alcohol and silver nanoparticles]. Вісник НТУУ “КПІ імені Ігоря Сікорського”. Серія: Хімічна інженерія, екологія та ресурсозбереження, (3), 83–92.https://doi.org/10.20535/2617-9741.3.2024.312424

2023

1. Zaremba A, Zaremba P, Zahorodnia S. De novo designed inhibitor has high affinity to four variants of the RBD of S-glycoprotein of SARS-CoV-2 – an in silico study. Journal of Biomolecular Structure and Dynamics. 2023; 41 (19), 9389-9397. https://doi.org/10.1080/07391102.2022.2141886 
2. Naumenko K, Zahorodnia S, Pop CV, Rizun N. Antiviral activity of silver nanoparticles against the influenza A virus. Journal of Virus Eradication. 2023; 9(2),100330.https://doi.org/10.1016/j.jve.2023.100330
3. Povnitsa OY, Zahorodnia SD, Artiukh LO, Zahornyi MM, Ievtushenko AI. Photodynamic Treatment of Titanium Dioxide Nanoparticles is a Convenient Method of Adenoviral Inactivation. Microbiological journal. 2023; 85(3): 61—69. https://doi.org/10.15407/microbiolj85.03.061  
4. Zaremba P, Zaremba A, Naumenko K, Yelipashev M, Zahorodnia S. In vitro and in silico studies of the antiviral activity of polyhydrated fullerenes against influenza A (H1N1) virus. Sci Rep. 2023;13: 10879. https://doi.org/10.1038/s41598-023-38128-3
5. Karpyna V, Myroniuk L, Myroniuk D, Bugaiova M, Petrosian L, Bykov O, Olifan O,  Strelchuk V, Kolomys O, Romanyuk V, Naumenko K, Artiukh L, Povnitsa O, Zahorodnia S, Ievtushenko A. Photocatalysis and optical properties of ZnO nanostructures grown on Si, Au/Si and Ag/Si wafers by the MOCVD method. Chemistry, physics and surface technology. 2023;14(1): 83-92. https://doi.org/10.15407/hftp14.01.083
6. Zaremba AA, Zaremba PY, Zahorodnia SD. In silico study of HASDI (high-affinity selective DNA intercalator) as a new agent capable of highly selective recognition of the DNA sequence. Sci Rep. 2023;13: 5395. https://doi.org/10.1038/s41598-023-32595-4                                                         
7. Zahornyi MM, Lavrynenko OM, Pavlenko OYu, Povnitsa OYu, Artiukh LO, Naumenko KS, Zahorodnia SD, Ievtushenko AI.The antiviral activity of cerium and lanthanum nanooxides modified with silver. Chemistry, physics and surface technology. 2023; 14(2): 262-272. https://doi.org/10.15407/hftp14.02.262
8. Iatsenko A, Sych O, Synytsia A, Zaremba P,  Zahorodnia S, Nikolenko A, Tomila T, Bykov O. Structure and properties of biogenic hydroxyapatite bioceramics modified by graphene-like structures. Appl Nanosci.  2023; 13: 7477–7483. https://doi.org/10.1007/s13204-023-02927-x
9. Chumakov V,  Ostryzhnyi M,  Kharchenko O,  Naumenko K,  Zagorodnya S, Muraveinyk V,  Tarasevich A. Antiviral Efficiency of Pulse UV Sterilization. Research Advances in Microbiology and Biotechnology 2023;  6: 128-141. https://doi.org/10.9734/bpi/ramb/v6/5542C
10. Ievtushenko A, Dzhagan V, Khyzhun O, Baibara O, Bykov O, Zahornyi M, Yukhymchuk V, Valakh M, Zahn DRT, Naumenko K Zaremba, P, Zagorodnya S. The effect of Ag doping on the structure, optical, and electronic properties of ZnO nanostructures deposited by atmospheric pressure MOCVD on Ag/Si substrates. Semiconductor Science and Technology. 2023; 38(7): 075008. https://doi.org/10.1088/1361-6641/acd6b2
11. Yavorovsky OP, Riabovol VM, Zinchenko TO, Zahornyi MM, Ragulya AV, Lavrynenko OM, Povnitsa OYu. The impact of silver nanoparticle modification on the structure, photoactive, toxicological, and virucidal properties of anatase for use in biology and medicine. The world of medicine and biology. 2023; 4 (86): 181-186.      https://doi.org/10 26724/2079-8334-2023-4-86-181-186
12. Demchenko VL, Goncharenko LA, Riabov SV, Rybalchenko NP, Hnatiuk TT, Mozhaeva LL, Zaremba PYu, Naumenko KS, Artiukh LO, Zahorodnia SD, Rybalchenko TV, Kokhtych LM. Formation of silver-containing films based on polyelectrolyte complexes by sputtering deposition and their antimicrobial and antiviral activity. Polymer journal. 2023; 45(2): 135-143.  https://doi.org/10.15407/polymerj.45.02.135

2022

1. Artiukh L, Povnitsa O, Zagorodnia S, Pop CV, Rizun N. Effect of coated silver nanoparticles on cancerous us healthy cells. Journal of Toxicology. 2022; (2):1-8. https://doi.org/10.1155/2022/1519104
2. Povnitsa O, Bilyavska L, Pankivska Yu, Likhanov A, Dorovskyh A, Lysenko V, Lokshin M, Zahorodnia S.  In vitro antiviral activity of leaf extracts Plantago major, Plantago lanceolate, Rubua idaeus. Microbiological journal. 2022; 84(1): 44-56.  https://doi.org:10.15407/microbiolj84.01.044
3. Zahorodnia SD, Naumenko KS, Zaichenko OV, Zaremba PYu, Baranova GV, Holovan AV. Effect of metal nanoparticles on ebv-associated cell culture. Microbiological Journal. 2022; 84(5): 30. https://10.15407/microbiolj84.05.030
4. Chaika M, Zahorodnya S, Naumenko K, Pankivska Yu, Povnitsa O, Mukha Yu, Vityuk N, Dorovskih A, Lokshyn M, Lysenko V, Losovski V, Rusinchuk N. Virus deformation or destruction:size-dependence of antiviral and virulicidal activities of gold nanoparticles. Advances in Natural Sciences: Nanoscience and Nanotechnology. 2022; 13(3): 035008  DOI: 10.1088/2043-6262/ac879a
5. Zaremba AA, Zaremba PY, Muchnyk FV, Baranova GV, Zahorodnia SD. In silico Identification of a Viral Surface Glycoprotein Site Suitable for the Development of Low Molecular Weight Inhibitors for Various Variants of the SARS-CoV-2. Microbiological Journal. 2022; 84 (1): 34-43. https://doi.org/10.15407/microbiolj84.05.030
6. Artiukh L, Povnitsa O, Shermolovich Y, Siry S, Zahorodnia S. The Antiviral Activity of Trifluoromethylthiolane Derivatives. Chem. Proc. 2022; 12: 41. https://doi.org/10.3390/ecsoc-26-13643
7. Demchenko V, Mamunya I, Kobylinskyi S, Riabov S, Naumenko K, Zahorodnia S, Povnitsa O, Rybalchenko N, Iurzhenko M, Adamus G, Kowalczuk M. Structure-morphology-antimicrobial and antiviral activity relationship in silver-containing nanocomposites based on polylactide. Molecules. 2022; 27: 3769. https://doi.org:103390/molecules27123769/
8. Lavrynenko OM, Zahornyi MM, Vember VV, Pavlenko OYu, Lobunets TF, Kolomys OF, Povnitsa OYu, Biliavska LO, Naumenko KS, Zahorodnia SD, Garmasheva IL. Nanocomposites based on cerium, lanthanum, and titanium oxides doped with silver for biomedical application. Condesed matter Physics, 2022; 7. https://doi.org:/10.3390/
9. Demchenko V, Iurzhenko M, Naumenko K, Kobylinskyiі S, Riabov S, Demchenko O, Zahorodnia S, Rybalchenko N, Hnatiuk T, Rybalchenko T, Kowalczuk M, Adamus G. Green Synthesis of Silver-Containing Biomaterials with Effective Antimicrobial and Antiviral Activity. 2022 IEEE 12th International Conference Nanomaterials: Applications & Properties (NAP), Krakow, Poland, 2022; 1-6. https://10.1109/NAP55339.2022.9934471
10. Synytsia A, Zaremba P, Zahorodnia S, Sych O, Babutina T, Kondratenko I. Biogenic hydroxyapatite-based composites modified by magnetite and chitosan: bioresorption in physiological solution and cytotoxicity. Funct. Mater. 2022; 29(4): 506. https://doi.org/10.15407/fm29.04.506
11. Kowalczuk M, Demchenko V, Kobylinskyi S, Iurzhenko M, Riabov S, Vashchuk A, Rybalchenko N, Zahorodnia S, Naumenko K, Demchenko O, Adamus G. Nanocomposites based on polylactide and silver nanoparticles and their antimicrobial and antiviral applications. Reactive and Functional Polymers. 2022; 170: 105096. https://doi.org/10.1016/j.reactfunctpolym.2021.105096
12. Zaremba A, Zaremba P, Budzanivska I, Zahorodnia S. Patterns of the influence of vaccination on the dynamics of different SARS-CoV-2 variants spread. Two-year analysis. Visnyk of Taras Shevchenko National University of Kyiv. 2022; 2(89): 39-45.  https://doi.org/10.17721/1728.2748.2022.89.39-45
13. Zaremba AA, Zaremba PY, Platonov MO. De novo designed EBAI as a potential inhibitor of the viral protein BHRF1. Research in silico. Journal of Biomolecular Structure and Dynamics. 2022; 41(8): 3680-3685,  https://doi.org/10.1080/07391102.2022.2053746

2021

1. Povnitsa OYu, Biliavska LO, Pankivska YuB, Zagorodnya SD, Borshchevskaya MI. Anti-Adenovirus Activity of the Medical Intranasal Drug Nazoferon. Mikrobiol. Zhurnal. 2021; 83(2):73-81. https://doi.org/10.15407/microbiolj83.02.073
2. Zahornyi MM, Tyschenko NI, Lobunets TF, Kolomys OF, Strelchuk VV, Naumenko KS,   Biliavska LO,. Zahorodnia SD, Lavrynenko OM, Ievtushenko  AI.  The Ag Influence on the Surface States of TiO₂, Optical Activity and Its Cytotoxicity//Journal of Nano- and Electronic Physics. 2021; 13(6): 06009(5рр). https://doi.org/10.21272/jnep.13(6).06009
3. Sinelnikov S, Orel L, Kobrina L, Boiko V, Riabov S, Shtompel V, Povnitsa O, Zagorodnya S. Polymer matrices on the basis of polacrylamid and b-cyclodextrin-containing pseudorotaxane for prolonged drug release: Synthesis and properties.Journal of Applied Polymer Science. 2021; 138(23):50554.  https://doi.org/10.1002/app.50554
4. Chumakov V,  Ostryzhnyi M, Kharchenko O, Naumenko K, Zagorodnya S, Muraveinyk V, Tarasevich A. Pulse VV sterilization of viral infection. Euliver: Microbes and Microbial Techniques.2021; 8(2): 001

2020

1. Zagorodnya S, Naumenko K, Golovan A. Prediction of Biological Activity of Triazoles Derivatives. Mikrobiol.Zhurnal. 2020; 82(1): 51-61. https://doi.org/10.15407/microbiolj82.01.051
2. Загородня CД, Головань АВ, Науменко КС, Мучник ФВ, Баранова ГВ, Зелена ЛБ. Антивірусна активність екстрактів з диких трав проти вірусу Епштейна-Барр та індукція апоптозу у вірус Епштейна-Барр-позитивних лімфобластоїдних клітинах. Мікробіологічний журнал. 2020;82(4): 71-79. https://doi.org/10.15407/microbiolj82.04.071
3. Chumakov V,  Ostryzhnyi M, Kharchenko O, Naumenko K, Zagorodnya S, Muraveinyk V, Tarasevich A. Experimental investigation of pulse sterilization of viral infection// bioRxiv preprint 2020. doi: https://doi.org/10.1101/2020.12.16.423002
4. Stasevich MV, Zvarich VI, Novikov VP, Zagorodnyaya SD, Povnitsa OYu, Chaika MA, Nesterkina MV, Kravchenko IA, Druzhilovskii DS, Poroikov VV. 9,10-Anthraquinone Dithiocarbamates as Potential Pharmaceutical Substances with Pleiotropic Actions: Computerized Prediction of Biological Activity and Experimental Validation. Pharmaceutical Chemistry Journal 2020; 53: 905-913. https://doi.org/10.1007/s11094-020-02098-x
5. Костюкевич КВ, Христосенко Р В, Загородня С Д, Костюкевич СО, Коптюх АА, Крючин АА, Олексенко ПФ. Молекулярна діагностика на основі кутової спектроскопії поверхневих плазмонів. Реєстрація, зберігання і обробка даних, 2020; 22(3): 14-30.

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2008 – Honors of the National Academy of Sciences of Ukraine for young scientists “Talent. Inspiration. Work” (Zahorodnia S.D.)

2008 – Diploma of honor of the Presidium of the National Academy of Sciences of Ukraine (Nesterova N.V.)

2015 – Prize of the National Academy of Sciences of Ukraine (L.O. Bilyavska, A.V. Golovan)

2017 – Grant of the President of Ukraine to support scientific research of young scientists (L.O. Bilyavska)

2019 – Prize of the President of Ukraine for young scientists (L.O. Bilyavska, K.S. Naumenko)

2019 –  Prize of the Kyiv Mayor  (L.O. Bilyavska)

2019 – Prize-winning place in the final of the innovative development competition “Stairway to the Future: award for young scientists in the field of healthcare and pharmaceuticals”(Bilyavska L.O., Pankivska Yu.B.)

2023 – Prize of the Verkhovna Rada of Ukraine to young scientists (L.O. Artyukh, K.S. Naumenko, S.V. Zasukha)